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CRISPR-Cas: A Laboratory Manual. Jennifer Doudna

CRISPR-Cas-A-Laboratory.pdf
ISBN: 9781621821311 | 450 pages | 12 Mb

• CRISPR-Cas: A Laboratory Manual
• Jennifer Doudna
• Page: 450
• Format: pdf, ePub, fb2, mobi
• ISBN: 9781621821311
• Publisher: Cold Spring Harbor Laboratory Press

Download CRISPR-Cas: A Laboratory Manual

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CRISPR-Cas-based techniques are revolutionizing the way geneticists and molecular biologists modify DNA sequences and modulate gene expression in cells and organisms. This laboratory manual presents step-by-step protocols for applying this cutting-edge technology to any system of interest. Contributors describe approaches for designing guide RNAs that precisely target the gene of interest, delivering CRISPR-Cas reagents into cells (e.g., using AAV vectors), and selecting cells that harbor the desired changes. Strategies for scaling and multiplexing these procedures are also described, as are methods for optimizing CRISPR-Cas systems in common species and cell lines, from yeast to human.

Crispr/Cas: A Laboratory Manual PDF - IndicaLivros.com
Editora: Cold Spring Harbor Laboratory Press (29/02/2016); ISBN-10: 1621821307; ISBN-13: 9781621821304; Capa dura: 450 páginas  More Info - TBM BookManager
Cover image for Crispr-Cas · More Info. 9781621821xxx. Crispr-Cas: A Laboratory Manual | Paperback Publisher: Cold Spring Harbor Laboratory Press 2 - Cold Spring Harbor Lab Press
Budding Yeast: A Laboratory Manual Edited By Brenda Andrews, University of this Product · CRISPR-Cas: A Laboratory Manual Edited By Jennifer Doudna,  Genome engineering using the CRISPR-Cas9 - Zhang Lab @ MIT
and the RNA-guided CRISPR-Cas nuclease system18–25. The first two technologies use the Illumina user manual. Off-target indel analysis  Patent US8697359 - CRISPR-Cas systems and methods - Google
The CRISPR-Cas system of claim 15 , wherein the expression of two or and Maniatis, MOLECULAR CLONING: A LABORATORY MANUAL,  Patent US8697359 - CRISPR-Cas systems and methods - Google
The CRISPR-Cas system of claim 15 , wherein the expression of two or and Maniatis, MOLECULAR CLONING: A LABORATORY MANUAL,  The CRISPR web page - CSIC
Lluis Montoliu's lab at CNB-CSIC, Madrid, Spain. Multiplex genome engineering using CRISPR/Cas systems. Cong L, Ran FA, Cox D, Lin S,  CRISPR-Cas based Genome Editing | Thermo Fisher Scientific
The CRISPR-Cas systems use sequences derived from plasmids and phages to activate Cas endonucleases to neutralize those plasmids and phages via  E-CRISP Start
in any nucleotide sequence for use in CRISPR/Cas mediated genome editing. The design process incorporates different options of how CRISPR constructs can be used in experimental applications. Please cite: Heigwer, F. , Kerr, G. & Boutros, M. , E-CRISP: fast CRISPR target site Boutros lab, E-CRISP-Version 4.2 CSHLP America - Cover image - CRISPR/Cas: A Laboratory Manual
CSHLP America - Cover image - CRISPR/Cas: A Laboratory Manual. Transcription, Processing, and Function of CRISPR Cassettes in
While laboratory strains of Escherichia coli contain a functional CRISPR/Cas CRISPR cassettes are often associated with cas genes (Jansen et al., 2002b; Sambrook J, Fritsch EF, Maniatis T. Molecular Cloning: A Laboratory Manual. Addgene: CRISPR/Cas9 Guide
Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening.

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